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KMID : 0861020080230030126
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Korea Journal of Herbology
2008 Volume.23 No. 3 p.126 ~ p.134
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Inhibitory Effect of Extract from Ostericum koreanum on LPS-induced Proinflammatory Cytokines Production in RAW264.7 Cells
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Park Hee-Je
Bae Gi-Sang
Kim Do-Yun
Seo Sang-Wan
Park Kyung-Bae
Kim Byung-Jin
Song Je-Moon
Lee Kyung-Yong
Na Cheol
Shin Byung-Cheul
Park Sung-Joo
Song Ho-Joon
Hwang Sung-Yeon
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Abstract
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Objectives : The present study was designed to investigate whether Ostericum koreanum (OK) could regulate lipopolysaccharide (LPS)-induced inflammatory response in vitro and in vivo.
Methods : To evaluate of anti-inflammatory effect of OK, we examined Nitric oxide (NO), proinflammatory cytokines production in LPS-stimulated RAW264.7 cells. Furthermore, we checked molecular mechanism especially in the phosphorylation of mitogen-activated protein kinases (MAPKs) and the degradation of inhibitory kappa B a (Ik-Bα) using western blot and also investigated survival of mice in LPS-mediated endotoxin shock.
Results : 1. Extract from OK itself have weak cytotoxic effect on RAW264.7 cells. Extract from OK inhibited LPS-induced NO, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and IL-10 production in RAW264.7 cells. 2. OK inhibited the phosphorylation of MAPKs, such as p38, extracelluar signal-regulated kinase (ERK1/2) and c-Jun NH2-terminal kinase (JNK) and also the degradation of Iκ-Bα in the LPS-stimulated RAW264.7 cells 3. OK did not inhibit LPS-induced endotoxin shock.
Conclusions : OK down-regulated LPS-induced NO and cytokines production through suppressing activation of MAPKs and degradation of Ik-Bα. Our results suggested that OK may be a beneficial drug against inflammatory diseases.
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KEYWORD
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Ostericum koreanum, OK, Lipopolysaccharide, LPS, inflammation, endotoxin shock
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